Plant Transcription Factor Database
Previous version: v3.0
Tarenaya hassleriana
SBP Family
Species TF ID Description
XP_010523498.1SBP family protein
XP_010524403.1SBP family protein
XP_010524772.1SBP family protein
XP_010524774.1SBP family protein
XP_010530548.1SBP family protein
XP_010534402.1SBP family protein
XP_010534410.1SBP family protein
XP_010536613.1SBP family protein
XP_010536615.1SBP family protein
XP_010536616.1SBP family protein
XP_010537252.1SBP family protein
XP_010538149.1SBP family protein
XP_010538150.1SBP family protein
XP_010538151.1SBP family protein
XP_010538152.1SBP family protein
XP_010538153.1SBP family protein
XP_010539753.1SBP family protein
XP_010539755.1SBP family protein
XP_010540239.1SBP family protein
XP_010542291.1SBP family protein
XP_010545847.1SBP family protein
XP_010549292.1SBP family protein
XP_010549293.1SBP family protein
XP_010549294.1SBP family protein
XP_010549337.1SBP family protein
XP_010551362.1SBP family protein
XP_010551363.1SBP family protein
XP_010551364.1SBP family protein
XP_010554861.1SBP family protein
XP_010555557.1SBP family protein
XP_010555558.1SBP family protein
XP_010555559.1SBP family protein
XP_010556129.1SBP family protein
XP_010556130.1SBP family protein
XP_010556131.1SBP family protein
XP_010556133.1SBP family protein
XP_010556134.1SBP family protein
XP_010556135.1SBP family protein
XP_010558510.1SBP family protein
XP_010559169.1SBP family protein
XP_010559170.1SBP family protein
XP_010559171.1SBP family protein
XP_010559216.1SBP family protein
SBP Family Introduction

SQUAMOSA promoter binding proteins (SBPs) form a major family of plant-specific transcription factors related to flower development. Although SBPs are heterogeneous in primary structure, they share a highly conserved DNA-binding domain (DBD) that has been suggested to be zinc binding. Here we report the NMR solution structures of DBDs of two SBPs of Arabidopsis thaliana, SPL4 and SPL7. The two share essentially the same structural features. Each structure contains two zinc-binding sites consisting of eight Cys or His residues in a Cys3HisCys2HisCys or Cys6HisCys sequence motif in which the first four residues coordinate to one zinc and the last four coordinate to the other. These structures are dissimilar to other known zinc-binding structures, and thus represent a novel type of zinc-binding motif. The electrostatic profile on the surface suggested that a continuous region, including all the conserved basic residues, is involved in the DNA binding, the mode of which is likely to be novel as well.

Yamasaki K, Kigawa T, Inoue M, Tateno M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Nunokawa E, Ishizuka Y, Terada T, Shirouzu M, Osanai T, Tanaka A, Seki M, Shinozaki K, Yokoyama S.
A novel zinc-binding motif revealed by solution structures of DNA-binding domains of Arabidopsis SBP-family transcription factors
J Mol Biol. 2004 Mar 12;337(1):49-63.
PMID: 15001351

SBP-box genes were first characterized as SQUAMOSA promoter binding proteins (SBPs) to regulate the expression of MADS-box genes in early flower development of A. majus (Klein et al., 1996). Since then, SBP-box genes have been identified in many plants including green alga, moss, silver birch, A. majus, Arabidopsis and maize. They play critical roles in regulating flower and fruit development as well as other physiological processes ([Moreno et al., 1997],[Eriksson et al., 2004], [Lannenpaa et al., 2004], [Arazi et al., 2005] and [Kropat et al., 2005]). It has been reported that Arabidopsis SPL3, SPL8 and SPL14 involves in flowering, sporogenesis, GA signaling and toxin resistance ([Cardon et al., 1997], [Unte et al., 2003], [Stone et al., 2005] and [Zhang et al., 2006]) while maize tga1 and tomato LeSPL-CNR affect fruit development ([Wang et al., 2005a] and [Manning et al., 2006]).

SBP-box genes encode proteins sharing a conserved DNA-binding domain of 79 amino acid residues. It has been proved that the DNA-binding domain of SBP-box genes is necessary and sufficient to bind to a palindromic GTAC core motif ([Klein et al., 1996], [Cardon et al., 1997], [Cardon et al., 1999], [Lannenpaa et al., 2004] and [Birkenbihl et al., 2005]). Studies on the NMR solution structure of the fragment of Arabidopsis SPL4 and SPL7 revealed that the DNA-binding domain of SBPs consisted of two separate zinc-binding sites. One zinc finger is C3H or C4 and the other is C2HC (Yamasaki et al., 2004 K. Yamasaki et al.,).

Guo AY, Zhu QH, Gu X, Ge S, Yang J, Luo J.
Genome-wide identification and evolutionary analysis of the plant specific SBP-box transcription factor family.
Gene. 2008 Jul 15;418(1-2):1-8.
PMID: 18495384